《中国神经再生研究（英文版）》杂志在2013年1月第3期出版的一项关于“Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor nanospheres”的研究发现，以下腰骶部背根神经节的中央突受压缢制备犬急性多重马尾压缢模型模拟临床马尾综合征，鞘内注射可吸收缓释聚(乳酸-羟基乙酸)-脑源性神经营养因子纳米微球后，可提高急性多重马尾压缢模型犬背根神经节感觉神经元内脑源性神经营养因子的表达，减轻神经元变性。文章作者认为，鞘内注射持续缓慢释放的脑源性神经营养因子纳米粒，可促进急性重度马尾综合征犬损伤神经结构及功能的修复。（生物谷Bioon.com）
DOI:10.3969/j.issn.1673-5374.2013.03.005
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Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles
Junming Tan1, Jiangang Shi2, Guodong Shi2, Yanling Liu3, Xiaohong Liu3, Chaoyang Wang1, Dechun Chen1, Shunming Xing1, Lianbing Shen1, Lianshun Jia2, Xiaojian Ye2, Hailong He2, Jiashun Li2
This study established a dog model of acute multiple cauda equina constriction by experimental constriction injury (48 hours) of the lumbosacral central processes in dorsal root ganglia neurons. The repair effect of intrathecal injection of brain-derived neurotrophic factor with 15 mg encapsulated biodegradable poly(lactide-co-glycolide) nanoparticles on this injury was then analyzed. Dorsal root ganglion cells (L7) of all experimental dogs were analyzed using hematoxylin-eosin staining and immunohistochemistry at 1, 2 and 4 weeks following model induction. Intrathecal injection of brain-derived neurotrophic factor can relieve degeneration and inflammation, and elevate the expression of brain-derived neurotrophic factor in sensory neurons of compressed dorsal root ganglion. Simultaneously, intrathecal injection of brain-derived neurotrophic factor obviously improved neurological function in the dog model of acute multiple cauda equina constriction. Results verified that sustained intraspinal delivery of brain-derived neurotrophic factor encapsulated in biodegradable nanoparticles promoted the repair of histomorphology and function of neurons within the dorsal root ganglia in dogs with acute and severe cauda equina syndrome.
